Radiological Study Of Mineralization Of Third Molars For Estimation Of  Age Using Demirjian Method

Authors

  • Avinash Babasaheb Andhale Assistant Professor, Department of Forensic Medicine, MGM Medical College, Vashi, Navi Mumbai. Author
  • Shailesh Mohite Dean, TNMC & BYL Nair Ch. Hospital, Mumbai. Author
  • Freny Karjodkar Ex Professor & Head, Department of Oral Medicine and Radiology, Nair Hospital Dental College, Mumbai Author
  • Pawan Sabale Professor Additional, Department of Forensic Medicine, TNMC & BYL Nair Ch. Hospital, Mumbai. Author

DOI:

https://doi.org/10.48165/jfmt.2025.42.4.05

Keywords:

Age estimation, Third molar mineralization, Demirjian method, Orthopantomogram, Forensic

Abstract

Accurate age estimation is necessary in forensic practice, particularly during late adolescence when legal distinctions  between juvenile and adult status carry significant implications. Dental parameters are widely regarded as reliable indi cators of age, as tooth development is minimally affected by environmental and systemic factors. Among these, third  molar mineralization remains especially valuable in individuals between 15 and 25 years of age due to its prolonged and  variable developmental timeline. This radiological study was undertaken to evaluate the relationship between third molar mineralization stages and  chronological age using Demirjian’s method, and to assess possible variations related to sex. The study included 200 indi viduals (100 males and 100 females) aged 15–25 years, all of whom were born and residing in Mumbai. Chronological  age was confirmed using official birth records. Orthopantomograms were obtained for each subject, and all four third  molars were assessed according to Demirjian’s eight-stage mineralization system. Statistical analysis was performed using  SPSS software. Early mineralization stages (A–C) were not observed in the study population. No statistically significant differences  were found between males and females or between the right and left sides in either jaw. Maxillary third molars showed  marginally earlier mineralization compared to mandibular molars. A strong positive correlation was observed between  mineralization stages and chronological age, allowing the derivation of regression equations for age estimation. Stage H  was consistently associated with attainment of adulthood. The study supports the relevance of Demirjian’s method for age estimation in late adolescence and early adulthood,  where reliable documentary evidence is unavailable. 

 

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References

Cunha E, Baccino E, Martrille L, Ramsthaler F, Prieto J, Schuliar Y. The problem of aging human remains and living individuals: a review. Forensic Sci Int. 2009;193(1–3):1–13.

Ritz-Timme S, Cattaneo C, Collins MJ, Waite ER, Schütz HW, Kaatsch HJ, et al. Age estimation: the state of the art in relation to the specific demands of forensic practice. Int J Legal Med. 2000;113(3):129–136.

Kvaal SI, Solheim T. A non-destructive dental method for age estimation. J Forensic Odontostomatol. 1994;12(1):6–11.

Mincer HH, Harris EF, Berryman HE. The ABFO study of third molar development and its use as an estimator of chronological age. J Forensic Sci. 1993;38(2):379–390.

Olze A, van Niekerk P, Ishikawa T, Zhu BL, Schulz R, Maeda H. Comparative study on the effect of ethnicity on wisdom tooth eruption. Int J Legal Med. 2007;121(6):445–448.

Demirjian A, Goldstein H, Tanner JM. A new system of dental age assessment. Hum Biol. 1973;45(2):211–227.

Kullman L, Johanson G, Akesson L. Root development of the lower third molar and its relation to chronological age. Swed Dent J. 1992;16(4):161–167.

Rai B, Kaur J, Cingolani M, Ferrante L, Cameriere R. Age estimation in children by measurement of open apices in teeth: a European formula. Int J Legal Med. 2007;121(6):449–453.

Bhat VS, Kamath GP. Age estimation from root development of mandibular third molars in comparison with skeletal maturity of wrist joint. Am J Forensic Med Pathol. 2007;28(3):238–241.

Schmeling A, Grundmann C, Fuhrmann A, Kaatsch HJ, Knell B, Ramsthaler F, et al. Criteria for age estimation in living individuals. Int J Legal Med. 2008;122(6):457–460.

Levesque GY, Demirjian A, Tanguay R. Sexual dimorphism in the development, emergence, and agenesis of the mandibular third molar. J Dent Res. 1981;60(10):1735–1741.

Harris EF, Clark LL. An epidemiological study of third molar agenesis in American Black and White people. Angle Orthod. 2008;78(5):794–798.

Celikoglu M, Miloglu O, Kazanci F. Frequency of agenesis, impaction, angulation, and related pathologic changes of third molar teeth in orthodontic patients. J Oral Maxillofac Surg. 2010;68(5):990–995

Hassanali J, Odhiambo JW. Position of third permanent molars in Kenyan Africans. East Afr Med J. 1981;58(1):6–11.

Sandhu SS, Bansal H, Monga P, Bhandari R. Study of eruption pattern of third molars in Punjabi population. J Forensic Dent Sci. 2013;5(1):5–9.

Arany S, Iino M, Yoshioka N. Radiographic survey of third molar development in relation to chronological age among Japanese juveniles. J Forensic Sci. 2004;49(3):534–538.

Gunst K, Mesotten K, Carbonez A, Willems G. Third molar root development in relation to chronological age: a large sample-sized retrospective study. Forensic Sci Int. 2003;136(1–3):52–57.

Nolla CM. The development of the permanent teeth. J Dent Child. 1960;27:254–266.

Köhler S, Schmelzle R, Loitz C, Püschel K. Development of wisdom teeth as a criterion of age determination. Ann Anat. 1994;176(4):339–345.

Moorrees CF, Fanning EA, Hunt EE Jr. Age variation of formation stages for ten permanent teeth. J Dent Res. 1963;42:1490–1502.

Bai Y, Mao J, Zhu S, Yang T, Tang Y. Radiographic study of third molar development in relation to chronological age among young adults in Shandong Province, China. Int J Legal Med. 2010;124(3):227–233.

Garn SM, Lewis AB, Bonne B. Third molar formation and its developmental course. Angle Orthod. 1962;32:270–279.

Kullman L. Accuracy of two dental and one skeletal age estimation method in Swedish adolescents. Forensic Sci Int. 1995;75(2–3):225–236.

Darji P, Banker H, Patel S, Dinkar A, Sahayata V. Age estimation using Demirjian’s method in Gujarati population: a comparative study. J Forensic Dent Sci. 2014;6(3):191–195.

Patil ST, Sarode SC, Sarode GS, Gadbail AR, Gondivkar SM, Karmarkar S. Radiographic assessment of third molar development using Demirjian method for age estimation in an Indian population. J Forensic Dent Sci. 2017;9(3):131–136.

Kanchan T, Krishan K, Pradeep Kumar G, Gupta A, Acharya J. Dental age estimation using Demirjian’s method in a South Indian population. J Forensic Leg Med. 2015;36:26–30.

De Angelis D, Gibelli D, Gaudio D, Cipriani M, Sforza C, Cattaneo C. Third molar development and its use in forensic age estimation: a radiological study using Demirjian staging. Forensic Sci Int. 2014;236:1–6.

Galić I, Vodanović M, Cameriere R, Nakaš E, Galić E, Selimović E, et al. Accuracy of Demirjian, Willems and Cameriere methods in dental age estimation on third molars: a systematic review. Forensic Sci Int. 2015;257:1–13.

Published

2026-02-07

How to Cite

Babasaheb Andhale, A., Mohite, S., Karjodkar, F., & Sabale, P. (2026). Radiological Study Of Mineralization Of Third Molars For Estimation Of  Age Using Demirjian Method. Journal of Forensic Medicine and Toxicology, 42(4), 22-28. https://doi.org/10.48165/jfmt.2025.42.4.05